		Technical Data
A0650-01L	ACK1, N-terminal GST tag, Recombinant, Human (TNK2, Tyrosine Kinase, non-receptor 2, Tyrosine Kinase non-receptor protein 2, ACK, ACK1, Activated CDC42 kinase 1, FLJ44758, FLJ45547, p21cdc42Hs)	10ug

Molecular Biology

Storage: -70°CShipping: Dry Ice

Source:
Recombinant corresponding to full length human rAck1 with N-terminal GTS tag expressed in Sf9 insect cell systems (NM_005781).

Specific Activity:
88pmol/min/mg Assay conditions: Add 50ul reaction mix (ACK1 diluted in 1x kinase buffer
supplemented with 0.2mM ATP) to wells coated with substrate. Incubate for 1hour. Add Europeum-labeled anti p-Tyr antibody and incubate for 1hour. Time resolved fluorescence measured at RT at lambda-exc=330±40nm and lambda-em=620±10nm.

Applications:
Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
68kD

Source: Sf9 cell
Purity: Purified 70%
Form: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% tween-20, 3mM DTT, 20% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eley, L. et al., Biochem. Biophys. Res. Commun. 371 (4), 877-882 (2008). 2. Grovdal, L.M. et al., Exp. Cell Res. 314 (6), 1292-1300 (2008).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.