		Technical Data
A0660-02G	ACO2 (Aconitate Hydratase, Mitochondrial, Citrate Hydro-lyase, Aconitase)

Description:
Aconitase 2 is an enzyme of the TCA cycle and is encoded in the nucleus and active within the mitochondrion. Aconitase catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. Isoforms of ACO2 have been reported but are not well characterized.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:16,000
Western Blot: 0.01-0.03ug/ml, observed in human heart lysates on ~90kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-QDTYQHPPKDSSGQH, from human Aconitase 2, at the internal region of the protein (NP_001089.1).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Specificity:

Recognizes human Aconitase 2. Species sequence homology: Bovine, canine, mouse, porcine and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Bota DA, Davies KJ. Lon protease preferentially degrades oxidized mitochondrial aconitase by an ATP-stimulated mechanism. Nat Cell Biol. 2002 Sep;4(9):674-80.