Technical Data
Acrolein, Conjugated
Acrolein is the simplest unsaturated aldehyde. It is produced widely but is most often immediately reacted with other products due to its instability and toxicity. It has a piercing, disagreeable, acrid smell similar to that of burning fat.

Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:5000-1:10,000
Immunohistochemistry: 1:500-1:1000
Western Blot: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Using a conjugate Acrolein-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:

Compound Crossreactivity ratio(a)
Acrolein-BSA 1
Malondialdehyde-BSA 1:55
Glyoxal-BSA 1:>50,000
Formaldehyde-BSA 1:>50,000
Glutaraldehyde-BSA 1:>50,000

(a): Acrolein-BSA concentration/unconjugated or conjugated analogs concentration at half displacement.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ug-20CBlue IceRabbit
Synthetic acrolein conjugated to BSA.
Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Reconstitute with 100ul sterile dH2O.
Recognizes conjugated Acrolein. See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Shen J., Yang X., Dong A., Petters R.M., Peng Y-W, Wong F. and Campochiaro P.A. J. of Cellular Physiology (2005) 203:457-464. 2. Komeima K, Rogers BS, Lu L, Campochiaro PA. Proc Natl Acad Sci USA. (2006) 103(30):11300-05. 3. Dorrell MI, Aguilar E, Jacobson R, Yanes O, Gariano R, Heckenlively J, Banin E, Ramirex GA, Gasmi M, Bird A, Siuzdak G, Friedlader, M. The Journal of Clinical Investigation (2009) 119(3):611-623. 4. Daiki R, Nishimura K, Ishii I, Omura T, Okuyama S, Kashiwagi K, Igarashi K. Stroke (2009) 40:1-6. 5. Hamann K, Durkes A, Oyuand H, Uchida K, Pond A, Shi R. Critical role of acrolein in secondary injury following ex vivo spinal cord trauma. J Neurochem. (2008) 107(3), 712-21.