Technical Data
Acrolein, Conjugated
Acrolein is the simplest unsaturated aldehyde. It is produced widely but is most often immediately reacted with other products due to its instability and toxicity. It has a piercing, disagreeable, acrid smell similar to that of burning fat.

Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Using a conjugate Acrolein-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:

Compound Crossreactivity ratio(a)
Acrolein-PC 1
Malondialdehyde-PC 1:>55
Glyoxal-PC 1:>50,000
Formaldehyde-PC 1:>50,000
Glutaraldehyde-PC 1:>50,000

(a): Acrolein-PC concentration/unconjugated or conjugated analogs concentration at half displacement.
(PC): Protein carrier

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug-20CBlue IceRabbit
~1mg/ml (after reconstitution)
Synthetic acrolein conjugated to protein carrier (PC).
Antiserum previously preabsobed on protein carriers and purified.
Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Reconstitute with 100ul sterile ddH2O.
Recognizes conjugated Acrolein. See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Shen J., Yang X., Dong A., Petters R.M., Peng Y-W, Wong F. and Campochiaro P.A. J. of Cellular Physiology (2005) 203:457-464. 2. Komeima K, Rogers BS, Lu L, Campochiaro PA. Proc Natl Acad Sci USA. (2006) 103(30):11300-05. 3. Dorrell MI, Aguilar E, Jacobson R, Yanes O, Gariano R, Heckenlively J, Banin E, Ramirex GA, Gasmi M, Bird A, Siuzdak G, Friedlader, M. The Journal of Clinical Investigation (2009) 119(3):611-623. 4. Daiki R, Nishimura K, Ishii I, Omura T, Okuyama S, Kashiwagi K, Igarashi K. Stroke (2009) 40:1-6. 5. Hamann K, Durkes A, Oyuand H, Uchida K, Pond A, Shi R. Critical role of acrolein in secondary injury following ex vivo spinal cord trauma. J Neurochem. (2008) 107(3), 712-21.