Technical Data
A0700-02A
Acrosin (Acrosin precursor, ACRS, ACR-2)
Description:
ACR is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18J160Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IcePorcineMouse
Concentration:
~1mg/ml
Immunogen:
Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, containing 15mM sodium azide
Specificity:
Reacts with various forms of porcine acrosin (55, 53, 45 and 35kD) in the acrosome of undamaged spermatozoa. Does not crossreact with bovine, canine or human. Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
This product has been used in:Moos J et al. Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction. Biol Reprod 49:408-15 (1993). Peknicova J & Moos J Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test. Andrologia 22:427-35 (1990).