Technical Data
A0725-02H
Acrp30 (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)
Description:
Human Adiponectin, also referred to as AdipoQ, Acrp30, apm-1 or GBP28, is a secreted protein expressed exclusively in differentiated adipocyte (adipokine). Adiponectin contains a modular structure comprising an N-terminal collagenous domain followed by a C-terminal globular domain (gAcrp30). Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and decreased levels are associated with insulin resistance and hyperinsulinemia.

Hybridoma:
Derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Acrp30 protein.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k7G7Affinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant human adiponectin (15-244aa) purified from E. coli
Purity:
Purified from mouse ascitic fluids by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% Thimerosal.
Specificity:
Recognizes human Adiponectin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Maeda K., et al. (1996) Biochem Biophys Res Commun. 221:286-9. 2. Berg AH., et al. (2001) Nat Med; 7:947-53. 3. Berg AH., et al. (2002) Trends Endocrinol Metab. 13: 84-89. 4. Yamauchi T et al., (2002) Nat Med. 8:1288-95.