Technical Data
A0725-02N
Acrp30 (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)
Description:
Acrp30 is a 244 amino acid major adipokine secreted into the bloodstream from adipose tissue to modulate metabolism, including glucose regulation and fatty acid catabolism. Unlike most other adipokines, adiponectin is secreted exclusively by differentiating adipocytes at reduced levels during obesity. The serum level of adiponectin is inversely correlated with BMI (body mass index) of an individual and has an anti-i nflammatory action, playing an important role in type II diabetes (insulin sensitivity) and atherosclerosis.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000. ~64kD band observed in mouse liver cell lysates.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG110B75Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to aa15-244 of human adiponectin expressed in E. coli.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes the collagenlike domain of human Adiponectin. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.