Technical Data
A0725-55
Acrp30, Mouse, BioAssay™ ELISA Kit (30kD Adipocyte Complement-related Protein, Adipocyte Complement-related 30kD Protein, ACDC, Adipocyte C1q and Collagen Domain Containing Protein, Adipocyte-specific Protein AdipoQ, Adiponectin, adipo, ADIPOQ, APM1, APN,
1Kit
Kits and Assays Storage: 4°CShipping: Blue Ice
Adipose tissue secretes a number of biologically active soluble factors (collectively named adipocytokines) that regulate glucose and fatty acid metabolism. Measurement of serum adiponectin levels gives us important information on the role of adiponectin in regulation of glucose and/or lipid metabolism. This Mouse Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in mouse serum, plasma or various tissue or cell culture supernatants. In the assay, polyclonal antibody specific for mouse adiponectin has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells and adiponectin present is bound by immobilized antibody. The bound adiponectin is then captured by anti-rat adiponectin monoclonal antibody. With HRP conjugated mouse IgG and a HRP substrate, the colors developed in proportion to the bound adiponectin, can be easily measured by ELISA plate reader.

Kit Components:
1) Antibody coated 96-well plate
2) 5X Wash concentrate, 100ml
3) 5X Diluent, 50ml
4) Secondary antibody, 12ml
5) 100X Detector, 150ul
6) Standard, recombinant mouse adiponectin (16ng), 1 vial, lyophilized
7) QC sample= positive control having 16-22ug/ml of mouse serum adiponectin
8) Substrate I, 6ml
9) Substrate II, 6ml
10) Stop solution (phosphoric acid), 12ml

Storage and Stability:
Store at 4°C. The reagents must be brought up to RT before use. Diluted wash solution may be stored at RT for up to one month. Do not expose the reagents to temperature above 25ºC. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.