Technical Data
Adipogenesis BioAssay™ Kit
Kits and Assays Storage: -20°CShipping: Blue Ice
Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured. The adipogenesis assay kit quantifies triglyceride accumulation in cells and tissues. In the assay, triglycerides are efficiently solubilized then hydrolyzed to glycerol which is subsequently oxidized to convert the probe to generate color (lambda-max = 570nm) and fluorescence (Ex/Em = 535/587nm). The kit can detect triglyceride in as few as a thousand or less differentiated 3T3-L1 cells with triglyceride detection linear range 0.2 to 10nmol. The high detection sensitivity and the convenient microplate assay format make the kit a convenient tool for studying the effect of adipogenesis inducers or inhibitors, or to screen drugs.

Kit Components:
*Adipogenesis Assay Buffer 1x25ml
Lipid Extraction Solution 1x10ml
Adipogenesis Probe (in DMSO solution) 1x200ul
Lipase (lyophilized) 1x1vial
Adipogenesis Enzyme Mix (lyophilized) 1x1vial
Triglyceride Standard (1mM) 1x0.3ml

Storage and Stability:
Store kit at –20°C, protect from light. Warm *Adipogenesis Assay Buffer to RT before use. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.