Technical Data
A0750-06C
ACT (Activator of cAMP Responsive Element Modulator (CREM) in Testis, Activator of CREM in Testis, dJ393D12.2, Four and a Half LIM Domains Protein 5, FHL5, FHL-5 Protein, FLJ33049, KIAA0776)
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Technical Data |
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A0750-06C |
ACT (Activator of cAMP Responsive Element Modulator (CREM) in Testis, Activator of CREM in Testis, dJ393D12.2, Four and a Half LIM Domains Protein 5, FHL5, FHL-5 Protein, FLJ33049, KIAA0776) |
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Description: FHL proteins are tissue specific cofactors of CREB and CREM and participate in regulation of various physiological processes. These proteins are characterized by the presence of 4 LIM domains and a LIM-half domain at the N-terminus. ACT, a 33kD protein, is a novel member of this family. It is specifically expressed in male testis and consists of a central activation domain. It is coordinately expressed with CREM and plays a vital role in spermatogenesis. It binds specifically to CREM and powerfully stimulates its transcriptional activation, essential for differentiation of spermatids into mature spermatozoa. It activates CREM in a phosphorylation- and CBP-independent manner. Protein-protein interactions with a Kinesin KIF17B regulate ACT activity in mouse. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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