Technical Data
A0758-01E
ACTH, NT (Adrenocorticotropic Hormone, Adrenocorticotropin, Corticotropin)
Description:
ACTH which, as a synthetic analogue of naturally-occurring Adrenocorticotropic Hormone, can be used to measure adrenal reserve and for the diagnosis of adrenal insufficiency by acute adrenocortical stimulation. ACTH, released from the anterior pituitary gland in response to corticotropin-releasing hormone from the hypothalamus, acts on the adrenal cortex to stimulate the production of corticosteroids such as cortisol, involved in the response to stress. Studies have shown that the administration of 1-24 ACTH increases blood pressure, believed to be attributed to an increase in ACTH-stimulated cortisol secretion, in association with increased cardiac output.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1:100-1:200 Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG157Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human ACTH
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human ACTH (aa1-24). Does not recognise ACTH (aa17-39). Species Crossreactivity: rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hatakeyama, H. et al. (2000) Functional adrenocorticotropic hormone receptor in cultured human vascular endothelial cells. Possible role in control of blood pressure. Hypertens. 36:862-865.