Technical Data
A0759-07
Actin
Description:
Actin is an abundant cytoskeletal protein found in all cells. The protein’s 42kD peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction. In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis and phagocytosis. Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:5000; HEK293 cell lysates were probed with A0759-07 and visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunocytochemistry (IF): 1:500; HeLa and NIH/3T3 cells were probed with A0759-07 and DAPI for nuclear staining. Visualized using an gG (Cy3) donkey anti-mouse. Positive staining pattern reflects both membrane and cytoplasmic staining.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b,k2Q1055Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceChickenMouse
Concentration:
~1mg/ml
Immunogen:
Purified chicken gizzard actin. Epitope: Appears to be located in the N-terminal two thirds of the actin molecule, possibly near aa50-70.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes all six isoforms of vertebrate actin, globular (G) and fillimentous (F) forms. This clone is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. Labels myotubes and stains myoblasts and fibroblasts. Does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. Species Crossreactivity: All vertebrates as well as Dictyostelium discoideum and Physarum polycephalum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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