Technical Data
A0759-08M
Actin
Description:
Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex. It has been reported that actin is hyperphosphorylated in primary breast tumors. It has also been shown that actin dysfunction leads to certain types of heart failure.

Applications:
Suitable for use in Indirect Immunofluorescence, ELISA, Western Blot Dot Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Indirect Immunofluorescence: 1:200-1:2000. Staining of cultured human or chicken fibroblasts
Western Blot: 1:500 using Human or chicken fibroblast extract. Band at ~ 42kD.
Immunohistochemistry (Paraffin): Methanol-fixed sections. Zinc-formalin, B5, ethanol, methacarn, Brunnel's or Bouin's solution can be used as fixatives. Can also be used on frozen sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a8J165Ascites
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Synthetic peptide, SGPSIVHALCP corresponding to human actin attached to Multiple Antigen Peptide backbone. Epitope: C-terminal. This epitope is conserved in all actin isoforms. Cellular Localization: Cytoplasmic
Purity:
Ascites
Form
Supplied as a liquid, 15mM sodium azide
Specificity:
Recognizes human actin. Species Crossreactivity: mouse, rat, bovine, chicken, canine, goat, guinea pig, porcine, rabbit, amphibian and sheep Does not react with the epitope on the N-terminal end of actin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Herman, I., Curr. Opin. Cell Biol., 5, 48 (1993). 2. Vanderkerckhove, J., and Weber, K., Eur. J. Biochem., 90, 451 (1978). 3. Drew, J., et al., Amer. J. Physiol., 260, C1332 (1991). 4. Lessard, J., Cell Motil. Cytoskel., 10, 349 (1988). 5. Sutoh, K., and Mabuchi, I., Biochemistry, 25, 6186 (1986).