Technical Data
Actin, Muscle Speci c
Actin is a major component of the cytoskeleton. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-Muscle-Specific Actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. This antibody is useful in the identification of rhabdoid cellular elements.

Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for immunohistochemical staining of formalin-fixed tissues.
Optimal dilutions to be determined by the researcher.

Positive Control:
Muscle or sarcoma

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,k3F10Affinity Purified
500ul-20CBlue IceHumanMouse
Purified actin. Cellular Localization: Cytoplasmic.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide., See A0760-22AX.
Recognizes alpha-smooth muscle, alpha-skeletal and alpha-cardiac (sarcomeric) isoforms of human actin. Reacts with tumors arising from smooth muscle (leiomyosarcomas) and skeletal muscle (rhabdomyosarcomas). Species Crossreactivity: mouse, rat, porcine, equine, bovine and canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tsukada, T., et al., Am. J. Pathol. 126: 51 (1987). 2. Tsukada, T., et. al., Am. J. Pathol. 127: 389 (1987).