Technical Data
A0760-22A
Actin, Muscle Speci c
Description:
Actin is a major component of the cytoskeleton. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-Muscle-Specific Actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. This antibody is useful in the identification of rhabdoid cellular elements.

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for immunohistochemical staining of formalin-fixed tissues.
Optimal dilutions to be determined by the researcher.

Positive Control:
Muscle or sarcoma

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k3F10Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Purified actin. Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide., See A0760-22AX.
Specificity:
Recognizes alpha-smooth muscle, alpha-skeletal and alpha-cardiac (sarcomeric) isoforms of human actin. Reacts with tumors arising from smooth muscle (leiomyosarcomas) and skeletal muscle (rhabdomyosarcomas). Species Crossreactivity: mouse, rat, porcine, equine, bovine and canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tsukada, T., et al., Am. J. Pathol. 126: 51 (1987). 2. Tsukada, T., et. al., Am. J. Pathol. 127: 389 (1987).