Technical Data
A0760-26
Actin, alpha, Smooth Muscle
Description:
Actin is a highly conserved skeletal protein which is in both muscle and nonmuscle cells. The mammalian tissues express at least 6 unique actin isotypes. Alpha isotype is found exclusively in muscle cells including alpha-skeletal, alpha-cardiac, and alpha and gamma smooth muscle actin. Beta and gamma nonmuscular actins have been observed in non-muscle cells.

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): 1:800 for 20 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.
Optimal dilutions to be determined by the researcher.

Positive Control:
Smooth muscle or leiomyosarcoma

Source:
Ascites

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k0.N.5Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.04mg/ml
Immunogen:
N-terminal decapeptide of the alpha smooth muscle isoform of Actin; acetylated at the N-terminus. Epitope: Acetyl group and the first 4 amino acids on the N-terminal end of the peptide chain of alpha-smooth Actin. Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See A0760-26X.
Specificity:
Recognizes human alpha-smooth muscle isoform of actin. Stains smooth muscle cells in vessel walls, gut wall and myometrium. Myoepithelial cells in breast and salivary gland are also stained. Reacts with tumors arising from smooth muscles and myoepithelial cells. Species Crossreactivity: baboon, bovine, rabbit, mouse, rat and chicken
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Skalli, O., et al., Journal of Cell Biology 103: 2787-2796 (1986). 2. Bochaton-Piallat, M.L., et al., Journal of Submicroscopic Cytology and Pathology 26: 1-8 (1994). 3. Cremona, O., et al., Journal of Histochemistry and Cytochemistry 42: 1221-1228 (1994). 4. Estes, J.M., Differentiation 56: 173-181 (1994). 5. Orlandi, A., et al., Arteriosclerosis and Thrombosis 14: 982-989 (1994).