Technical Data
A0760-26H
ACTA2 (Actin, alpha Smooth Muscle, Actin, Aortic Smooth Muscle, Alpha-actin-2, Cell Growth-inhibiting Gene 46 Protein, ACTSA, ACTVS, GIG46)
Description:
Actin is a highly conserved protein which participates in a wide variety of cellular functions in eucaryotes including muscle contraction, amoeboid movement, cytokinesis, and mitotic division. Humans have six actin isotypes; two striated muscle types or sarcomeric (alpha-skeletal and alpha-cardiac), two smooth muscle types and two cytoskeletal forms (beta and gamma-types). Each isotypes is coded by a separate gene and is expressed in a developmentally regulated and tissue-specific manner. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. The smooth muscle alpha-actin is expressed in vascular smooth muscle cells and fibroblast cells. Its expression is regulated by hormones, cell proliferation and repressed by pathological conditions like oncogenic transformation and artherosclerosis. It maps to 10q23.3 region of human chromosome. Northern blot analysis revealed that human smooth muscle cells express only a single 1.4 kilobase smooth muscle alpha actin transcript.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:200
Immunohistochemistry (frozen): 1:25-1:100
Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
Leiomyoma

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k11E6Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceMouse
Concentration:
Not determined
Immunogen:
N-terminal decapeptide of alpha-smooth muscle actin. Cellular Localization: Cytoplasmic
Purity:
Purified
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity:
Recognizes Actin, alpha smooth muscle. Species Crossreactivity: baboon, chicken, canine, frog, goat, human, mouse, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Skalli et al. Am J Cell Biol 103: 2787, 1986. 2. Skalli et al. Lab Invest 60: 275, 1989. 3. Skalli et al. J Histo Chem 37: 315, 1989. 4. O'Byrne et al. Br J cancer 82: 1427, 2000. 5. Come et al. Oncogene 16: 1299, 1998. 6. Reddy et al. The Journal of Biological Chemistry 265: 1683, 1990.