Technical Data
Actin, Smooth Muscle
Smooth muscle actin is expressed in normal smooth muscle and in myoepithelial cells, and also in tumors derived from these cells. Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 1:50- 1:100 for 30 min at RT (No special pretreatment is required for staining of formalin/paraffin tissues).
Optimal dilutions to be determined by the researcher.

Positive Control:
Smooth muscle of arteries, gut wall, uterus and leiomyosma

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
250ul-20CBlue IceHumanRabbit
A synthetic peptide from N-terminus of human alpha smooth muscle isoform of actin. Cellular Localization: Cytoplasmic
Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.
Recognizes human Actin, smooth muscle. Reacts with tumors arising from smooth muscles and myoepithelial cells. Stains smooth muscle cells in vessel walls, gut wall and myometrium. Myoepithelial cells in breast and salivary gland are also stained. Species Crossreactivity: baboon, bovine, rabbit, mouse, rat and chicken.Purified
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.