Technical Data
A0760-33A
Actin, Smooth Muscle (alpha-Actin 2, ACTA2)
Description:
Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex. It has been reported that actin is hyperphosphorylated in primary breast tumors. It has also been shown that actin dysfunction leads to certain types of heart failure. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Furthermore, expression of smooth muscle alpha actin is regulated by hormones and cell proliferation, and is altered by pathological conditions including oncogenic transformation and atherosclerosis.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:20
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:1000-1:2000. Only detects actin in smooth muscle.
Immunoprecipitation: 1:20
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k6Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to N-terminus of human Actin.
Purity:
Supernatant
Form
Supplied as a liquid in Tris-glyceine, sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human actin at ~42kD. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Herman, I.M. Actin isoforms. Curr. Opin. Cell Biol. 5: 48–55 (1993). 2. Bailly, M., et al. Curr Biol. 11: 620–625 (2001). 3. Lim, Y.P., et al. Clin. Cancer Res. 10: 3980–3987 (2004). 4. Olson, T.M., et al. Science 280: 750–752 (1998). 5. Miwa, T., et al. Mol Cell Biol. 11: 3296-3306 (1991).