Technical Data
Actin, beta
Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western Blot. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.5-1ug/ml. Useful as a loading control for a wide variety of cell extracts including human foreskin fibroblasts or chicken fibroblasts.
Immunohistochemistry (Paraffin and frozen): The epitope recognized by the antibody is resistant to paraffin or formalin-fixed tissue sections. Acetone fixation or B5, Methacarn, ethanol or Bouins solution may also be used as fixatives.
Immunofluorescence (IC): 10-40ug/ml on methanol/acetone fixed samples. Staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membranes associated dense-plaque.
Optimal dilutions to be determined by the researcher.

Positive Control:
Cultured human or chicken fibroblasts

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceMouse
Synthetic peptide corresponding to Ac-Asp-Asp-Asp-Ile-Ala-Ala- Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys from beta Actin at N-terminal, conjugated to KLH. Molecular Weight: 42kD.
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Recognizes beta Actin in a wide variety of tissues and species. Does not crossreact with adult cardiac or skeletal muscle or amoeba beta actin. Species Crossreactivity: human, bovine, sheep, porcine, rabbit, feline, canine, mouse, rat, guinea pig, chicken, fish (carp) and leech.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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