Technical Data
A0760-40
Actin, beta
Description:
Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western Blot. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.5-1ug/ml. Useful as a loading control for a wide variety of cell extracts including human foreskin fibroblasts or chicken fibroblasts.
Immunohistochemistry (Paraffin and frozen): The epitope recognized by the antibody is resistant to paraffin or formalin-fixed tissue sections. Acetone fixation or B5, Methacarn, ethanol or Bouin’s solution may also be used as fixatives.
Immunofluorescence (IC): 10-40ug/ml on methanol/acetone fixed samples. Staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membranes associated dense-plaque.
Optimal dilutions to be determined by the researcher.

Positive Control:
Cultured human or chicken fibroblasts

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12A2.1Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceXenopusMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa1-14, DDDIAALVIDNGSGL of Xenopus laevis beta Actin, conjugated to KLH. Epitope: N-terminal of the beta isoform of actin. Molecular Weight: 42kD.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity:
Recognizes Xenopus beta Actin in a wide variety of tissues and species. Does not crossreact with adult cardiac or skeletal muscle or amoeba beta actin. Species Crossreactivity: human, bovine, sheep, porcine, rabbit, feline, canine, mouse, rat, guinea pig, chicken, fish (carp) and leech.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application references: 1. Li, Q. et al., (2004) J. Biol. Chem. 279:52106-52116. 2. Morales, S.A. (2009) Investigative Ophthalmology and Visual Science. 50:462-469. 3. Morales, S. A. (2007) Experimental Eye 85: 790-798. 4. Morales, S.A. (2009) Invest Ophthalmol Vis Sci. 50:4949-4956. 5. Gort, E.H. et al., (2006) Oncogene 25:6123–6127. 6. Weidong, W. et al., (2010) Environ Health Perspect. 118; 982–987. 7. de Koning, P J .A., et al., (2011) PLoS ONE 6: e22645. doi:10.1371/journal.pone.0022645. 8. Kesic, M.J. et al., (2012) PLoS ONE 7: e35108. doi:10.1371/journal.pone.0035108 1. Gimona, M., et al., Cell Motil. Cytoskel. 27: 108-116 (1994). 2. North, A.J., et al., J. Cell Sci. 107: 445-455 (1994). 3. North, A.J., et al., J. Cell Sci. 107: 437-444 (1994). 4. Liao, J., et al., Biotechniques 28: 216-218 (2000). 5. Vaziri, H., et al., Cell 107: 149-159 (2001). 6. Ueda, T., et al., J. Biol. Chem. 277: 38,087-38,094 (2002).