		Technical Data
A0760-95B	ACTL6B, ACTL6A (Actin-like Protein 6A, 53kD BRG1-associated Factor A, Actin-related Protein Baf53a, ArpNbeta, BRG1-associated Factor 53A, BAF53A/ Actin-like Protein 6B, 53kD BRG1-associated Factor B, Actin-related Protein Baf53b, ArpNalpha, BRG1-associate

Description:
The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53kD subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix.

Applications:
Suitable for use in ELISA and Western Blot Other applications not tested.

Recommended Dilution:
ELISA: 1:64,000
Western Blot: 1-3ug/ml, observed in Hela lysates on ~48kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to YEEGGKQCVERKCP, from human ACTL6A/ACTL6B, at C-terminal (NP_004292.1, NP_817126.1; NP_057272.1).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Specificity:

Recognizes human ACTL6A/ACTL6B. Species sequence homology: Bovine, canine, mouse, porcine and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Harata M, Mochizuki R, Mizuno S. Two isoforms of a human actin-related protein show nuclear localization and mutually selective expression between brain and other tissues. Biosci Biotechnol Biochem. 1999 May;63(5):917-23.