Technical Data
A0760-95B
ACTL6B, ACTL6A (Actin-like Protein 6A, 53kD BRG1-associated Factor A, Actin-related Protein Baf53a, ArpNbeta, BRG1-associated Factor 53A, BAF53A/ Actin-like Protein 6B, 53kD BRG1-associated Factor B, Actin-related Protein Baf53b, ArpNalpha, BRG1-associate
Description:
The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53kD subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix.

Applications:
Suitable for use in ELISA and Western Blot Other applications not tested.

Recommended Dilution:
ELISA: 1:64,000
Western Blot: 1-3ug/ml, observed in Hela lysates on ~48kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to YEEGGKQCVERKCP, from human ACTL6A/ACTL6B, at C-terminal (NP_004292.1, NP_817126.1; NP_057272.1).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human ACTL6A/ACTL6B. Species sequence homology: Bovine, canine, mouse, porcine and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Harata M, Mochizuki R, Mizuno S. Two isoforms of a human actin-related protein show nuclear localization and mutually selective expression between brain and other tissues. Biosci Biotechnol Biochem. 1999 May;63(5):917-23.