		Technical Data
A0761-09G	ACTN2 (Alpha-actinin-2, Alpha-actinin Skeletal Muscle Isoform 2, F-actin Cross-linking Protein)

Description:
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:100-1:200
Western Blot: 1:500-1:3000
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Recombinant corresponding to human ACTN2.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.0, 0.01% thimerosal, 10% glycerol.

Specificity:

Recognizes human ACTN2. Species Crossreactivity: mouse. Species sequence homology: Rat (91%), bovine (99%), zebrafish (92%).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.