Technical Data
A0761-09H
ACTN2 (Alpha-actinin-2, Alpha-actinin Skeletal Muscle Isoform 2, F-actin Cross-linking Protein)
Description:
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Applications:
Suitable for use in Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:3000
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Immunofluorescence: 1:100-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to aa215-500 of human ACTN2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7, 0.01% thimerosal, 10% glycerol.
Specificity:
Recognizes human ACTN2. Species sequence homology: mouse (100%), rat (85%), bovine (84%), monkey (100%) and zebrafish (86%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.