		Technical Data
A0761-10	ACTN3, NT (Alpha-actinin-3, Alpha-actinin Skeletal Muscle Isoform 3, F-actin Cross-linking Protein)

Description:
Alpha-actinin is an actin-binding protein with multiple roles in different cell types. ACTN3 expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:20,000
Western Blot: 1:500-1:1000
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to human ACTN3.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

Specificity:

Recognizes human ACTN3. Species sequence homology: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.