Technical Data
A0855-89
Activin A Precursor
Description:
Activins are dimers consisting of two beta subunits. Several types of beta subunits have been described. The same beta subunits also occur in Inhibins as part of a heterodimer with an alpha subunit. The beta subunits are translated as a precursor form that includes an N-terminal pro region which is proteolytically cleaved to yield the mature C-terminal beta subunit.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human Activin A precursor is ~15ng/well.
Western Blot: 1- 2ug/ml. The detection limit for recombinant human Activin A precursor is ~5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse myeloma with B cells obtained from a mouse immunized with a DNA construct encoding the human Activin A pro region fused to mature human Activin beta B chain.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19H16Affinity Purified
SizeStorageShippingSourceHost
500ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
DNA construct encoding human Activin A precursor.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 1ml sterile PBS.
Specificity:
Recognizes human Activin A Precursor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.