Technical Data
A0855-90E
Activin A Receptor type IB (ACVR1B, Activin Receptor-like Kinase 4, Activin Receptor Type 1B Precursor, ACTR-IB, ACVRLK4, ALK4)
Description:
Activin RIB, also known as activin-like kinase 4 (ALK-4), is a transmembrane serine/threonine kinase that complexes with Activin type II receptors, Activin RII or Activin RIIB, to form the heteromeric signaling complex. Several members of the TGF-beta family, including the various Activins, Nodal, and GDF-1, have been shown to signal via the Activin RIB and Activin RII/IIB complex.

Applications:
Suitable for use in ELISA and Flow Cytometry. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1ug/ml
Flow Cytometry: 25ug/ml, 10ul labels 1-2.5 x 10e5 cells in 100-200ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse myeloma cells with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Activin type I receptor IB extracellular domain (aa24-126).

Storage and Stability:
Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2B9L800Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Purified, NS0-derived, recombinant human Activin type I receptor IB extracellular domain (aa24-126; Accession # P36896).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Specificity:
Recognizes human RIB.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.