		Technical Data
A0855-90F	Activin A Receptor Type IB (ACVR1B, Activin Receptor-like Kinase 4, Activin Receptor Type 1B Precursor, ACTR-IB, ACVRLK4, ALK4)

Description:
The activin A receptor type 1 (ACVR1) is a membrane associated serine/threonine kinase receptor that binds activin A and TGF-B superfaily growth factors, including bone morphogenetic protein 2 (BMP2) and BMP4, 6 and 7 (1-3). Functional activin receptors are composed of a pair of type I and type II activin receptor proteins. Following ligand binding, ACVR1 is phosphorylated by its type II binding partner, which allows recruitment and activation of receptor regulated SMAD (R-Smad) proteins (4). Phosphorylation of R-Smad proteins results in their translocation to the nucleus where R-Smads activate transcription of target genes (5). Mutations in the corresponding ACVR1 gene are associated with the genetic developmental disorders hereditary hemorrhagic telangiectasia and fibrodysplasia ossificans progressive (6,7).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to residues near the carboxy terminus of human ACVR1 protein (KLH-coupled).

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

Specificity:

Recognizes human ACVR1. Species crossreactivity: mouse, monkey.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Attisano, L. et al. (1993) Cell 75, 671-80, 2. Macías-Silva, M. et al. (1998) J Biol Chem 273, 25628-36, 3. Ho, C.C. and Bernard, D.J. (2009) Biol Reprod 81, 133-41, 4. Attisano, L. et al. (1996) Mol Cell Biol 16, 1066-73, 5. Attisano, L. and Wrana, J.L. (2000) Curr Opin Cell Biol 12, 235-43, 6. Shore, E.M. et al. (2006) Nat Genet 38, 525-7, 7. Letteboer, T.G. et al. (2005) Hum Genet 116, 8-16.