Technical Data
A0855-90F
Activin A Receptor Type IB (ACVR1B, Activin Receptor-like Kinase 4, Activin Receptor Type 1B Precursor, ACTR-IB, ACVRLK4, ALK4)
Description:
The activin A receptor type 1 (ACVR1) is a membrane associated serine/threonine kinase receptor that binds activin A and TGF-B superfaily growth factors, including bone morphogenetic protein 2 (BMP2) and BMP4, 6 and 7 (1-3). Functional activin receptors are composed of a pair of type I and type II activin receptor proteins. Following ligand binding, ACVR1 is phosphorylated by its type II binding partner, which allows recruitment and activation of receptor regulated SMAD (R-Smad) proteins (4). Phosphorylation of R-Smad proteins results in their translocation to the nucleus where R-Smads activate transcription of target genes (5). Mutations in the corresponding ACVR1 gene are associated with the genetic developmental disorders hereditary hemorrhagic telangiectasia and fibrodysplasia ossificans progressive (6,7).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues near the carboxy terminus of human ACVR1 protein (KLH-coupled).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.
Specificity:
Recognizes human ACVR1. Species crossreactivity: mouse, monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Attisano, L. et al. (1993) Cell 75, 671-80, 2. Macas-Silva, M. et al. (1998) J Biol Chem 273, 25628-36, 3. Ho, C.C. and Bernard, D.J. (2009) Biol Reprod 81, 133-41, 4. Attisano, L. et al. (1996) Mol Cell Biol 16, 1066-73, 5. Attisano, L. and Wrana, J.L. (2000) Curr Opin Cell Biol 12, 235-43, 6. Shore, E.M. et al. (2006) Nat Genet 38, 525-7, 7. Letteboer, T.G. et al. (2005) Hum Genet 116, 8-16.