Technical Data
Activin B, Recombinant, Human
Molecular Biology Storage: -20CShipping: Blue Ice
Activin B is a TGF- family member that exhibits a wide range of biological activities including regulation of embryogenesis, osteogenesis, hematopoiesis, reproductive physiology and hormone secretion from the hypothalamic, pituitary and gonadal glands. Activin B, like certain other members of the TGF- family, signals through the ActRII receptor (Activin Receptor type II). Human Activin B is a 26.0kD disulfide-linked homodimer consisting of two B chains, each containing 115 amino acid residues. *Manufactured using BTI-Tn-5B1-4 cells under license from the Boyce Thompson Institute for Plant Research, Inc

(BTI-Tn-5B1-4) High-5 Insect cells

Biological Activity:
The ED50 as determined by its ability to inhibit the proliferation of murine MPC-11 cells is <2ng/ml, corresponding to a specific activity of > 5x10e5 units/mg.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20C. Reconstituted product is stable for 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions may be made in PBS containing 2mg/ml albumin.

Country of Origin:
Source: (BTI-Tn-5B1-4) High-5 Insect cells
Purity: ~95% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug (1EU/ug).
Concentration: As reported
Form: Supplied as lyophilized powder from 10mM sodium citrate, pH 3.0, 5% trehalose.
Reconstitution: Reconstitute to a concentration of 0.5mg/ml in 10mM citric acid. Further dilutions may be made in PBS containing 2mg/ml albumin.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.