Technical Data
Activin Receptor Type IA (ALK-2, RIA)
Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I and two type II receptors. Unlike ACVR1B and ACVR1C, ACVR1, also known as activin receptor-like kinase 2 (ALK2), can not transduce activin-mediated signaling, but will transduce BMP and Mullerian inhibiting substance (MIS) group signaling. It is thought that ACVR1 also inhibits activin signaling by blocking the binding of activin to its type II receptor. Recent studies indicate that genetic variation in ACVR1 is associated with polycystic ovary syndrome, suggesting that ACVR1 signaling contributes to disturbed folliculogenesis in these patients. At least four isoforms of ACVR1 are known to exist. This antibody is predicted to have no crossreactivity to ACVR1B or ACVR1C.

Suitable for use in Flow Cytometry, Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 2.5ug/10e6 cells using PC-3 human prostate cancer cell line
Western Blot: 0.1ug/ml using recombinant human Activin RIA/ALK-2 Fc Chimera
Immunohistochemistry (Paraffin): 5-15ug/ml using immersion fixed paraffin-embedded sections of human astrocytoma and human placenta
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
~0.2mg/ml (after reconstitution)
Recombinant protein corresponding to Asp23-Glu123 of human Activin Receptor Type IA expressed in Sf21 cells (Q04771).
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Recognizes human Activin Receptor Type IA (RIA). Shows <1% crossreactivity with recombinant human Activin RIB, Activin RIIA and Activin RIIB.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.