		Technical Data
A0855-97A	ACVR2B, CT (V474) (Activin Receptor Type 2B, Activin Receptor Type IIB, ACTR-IIB)

Description:
Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases. ACVR2B (activin A type IIB receptor)displays a 3- to 4-fold higher affinity for the ligand than activin A type II receptor.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the C-terminal region of human ACVR2B (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human ACVR2B.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Harrison, C.A., et al., J. Biol. Chem. 279(27):28036-28044 (2004). 2.Martins da Silva, S.J., et al., Dev. Biol. 266(2):334-345 (2004). 3.Casagrandi, D., et al., Mol. Hum. Reprod. 9(4):199-203 (2003). 4.Shin, B.K., et al., J. Biol. Chem. 278(9):7607-7616 (2003). 5.Schneider-Kolsky, M.E., et al., Placenta 23(4):294-302 (2002).