Description:
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Immunoprecipitation 1:10-1:500
Western Blot 1:500
Immunocytochemistry/Immunofluorescence 1:200
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ug | | -20°C | Blue Ice | | Rabbit |
|
| Concentration: |
| ~0.75mg/ml |
| Immunogen: |
| Synthetic peptide: KSTTEATTQVPKEKTQDGSN[C]. Peptide was amidated for conjugation. |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| 0.02% Sodium Azide Supplied in antibody stabilization buffer |
| Specificity: |
| Recognizes drosphilia melanogaster Punt at ~63kD. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
Henderson KD et al. Cell fate specification in the Drosophila salivary gland: the integration of homeotic gene function with the DPP signaling cascade. Dev Biol 205:10-21 (1999). Xie T & Spradling AC decapentaplegic is essential for the maintenance and division of germline stem cells in the Drosophila ovary. Cell 94:251-60 (1998). Singer MA et al. Signaling through both type I DPP receptors is required for anterior-posterior patterning of the entire Drosophila wing. Development 124:79-89 (1997).
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