Technical Data
A0856-01
Punt
Description:
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunoprecipitation 1:10-1:500
Western Blot 1:500
Immunocytochemistry/Immunofluorescence 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRabbit
Concentration:
~0.75mg/ml
Immunogen:
Synthetic peptide: KSTTEATTQVPKEKTQDGSN[C]. Peptide was amidated for conjugation.
Purity:
Purified by immunoaffinity chromatography.
Form
0.02% Sodium Azide Supplied in antibody stabilization buffer
Specificity:
Recognizes drosphilia melanogaster Punt at ~63kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Henderson KD et al. Cell fate specification in the Drosophila salivary gland: the integration of homeotic gene function with the DPP signaling cascade. Dev Biol 205:10-21 (1999). Xie T & Spradling AC decapentaplegic is essential for the maintenance and division of germline stem cells in the Drosophila ovary. Cell 94:251-60 (1998). Singer MA et al. Signaling through both type I DPP receptors is required for anterior-posterior patterning of the entire Drosophila wing. Development 124:79-89 (1997).