Technical Data
Activin Receptor Type IIA (RIIA)
Activin isoforms and other members of the TGF­beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine­threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR­I(A), ActR­IB, ActR­II(A) and ActR­IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR­IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high­affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin, but will associate with the type II receptor­activin complex and initiate signal transduction. Besides the activin isoforms, ActR­II will also bind inhibin, BMP­2 and BMP­7 with lower affinities. ActR­I can also bind and form signaling complexes with the BMP­2/7­bound BMPR­II. Activin type II receptors are highly conserved. Human, mouse and rat type II activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type II receptors bind activin with high affinity, and are potent activin antagonists.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml using human testis tissue.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2a9H20Affinity Purified
500ug-20°CBlue IceHumanMouse
~0.5mg/ml (after reconstitution)
Recombinant corresponding to aa20-134 of human Activin RIIA expressed in Sf21 cells (P27037).
Purified by Protein A affinity chromatography.
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.
Recognizes human Activin RIIA. Does not crossreact with recombinant human Activin RIA, recombinant human Activin RIB or recombinant human Activin RIIB.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Attisano, L. et al. (1996) Mol. and Cell. Biol. 16:1066. 2. Woodruff, T.K. (1998) Biochem. Pharmacology, 55:953.