Technical Data
A0856-10A
Activin Receptor Type IIB (RIIB)
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Technical Data |
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A0856-10A |
Activin Receptor Type IIB (RIIB) |
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Description: Members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, Bmp7, Gdf1, Gdf3, Myostatin (Gdf8), and Gdf11. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated. Applications: Suitable for use in Neutralization, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Neutralization: ~1-3ug/ml will block 50% of the binding of 30ng/ml of recombinant human Activin A to immobilized recombinant human Act RIIB/Fc Chimera (100ul of a 2ug/ml solution was coated in each well) in an ELISA type assay. Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.1ng/well. Western Blot: 0.1-0.2ug/ml. The detection limit is ~1ng/lane under non-reducing and reducing conditions. Immunohistochemistry (Paraffin): 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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