Technical Data
Activin Receptor Type IIB (RIIB)
Members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, Bmp7, Gdf1, Gdf3, Myostatin (Gdf8), and Gdf11. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated.

Suitable for use in Neutralization, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Neutralization: ~1-3ug/ml will block 50% of the binding of 30ng/ml of recombinant human Activin A to immobilized recombinant human Act RIIB/Fc Chimera (100ul of a 2ug/ml solution was coated in each well) in an ELISA type assay.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.1ng/well.
Western Blot: 0.1-0.2ug/ml. The detection limit is ~1ng/lane under non-reducing and reducing conditions.
Immunohistochemistry (Paraffin): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
NS0-derived, recombinant human Activin Receptor Type IIB extracellular domain.
Purified by immunoaffinity chromatography. Endotoxin: <0.1EU/ug (LAL)
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Recognizes human Activin Receptor Type IIB (RIIB). Shows <1% crossreactivity with recombinant human Activin RIIA, recombinant human Activin RIA and recombinant human Activin RIB.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.