Technical Data
A0856-10C
Activin Receptor Type IIB (RIIB)
Description:
Members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, BMP-7, GDF-1, GDF-3, Myostatin (GDF-8), and GDF-11. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human Activin RIIB is ~2ng/well.
Western Blot: 1-2ug/ml. The detection limit for recombinant human Activin RIIB is ~20ng/lane under reducing and non-reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19H21Affinity Purified
SizeStorageShippingSourceHost
500ug4°CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to the extracellular domain of human Activin RIIB, expressed in NSO cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Specificity:
Recognizes recombinant human Activin RIIB. Shows ~5% crossreactivity with recombinant human Activin RIIA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.