		Technical Data
A0856-10D	Activin Receptor Type IIB (RIIB)

Description:
Members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, Bmp7, Gdf1, Gdf3, Myostatin (Gdf8), and Gdf11. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml using recombinant human Activin RIIB Fc Chimera.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	9H22	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml (after reconstitution)

Immunogen:

Recombinant protein corresponding to aa19-134 from human Activin RIIB, expressed in NSO cells (Q13705).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

Specificity:

Recognizes human Activin RIIB. Shows ~5% crossreactivity with recombinant human Activin RIIA.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.