Technical Data
A0857
ACTR (AIB1, Amplified in Breast Cancer-1, AIB1, RAC3, TRAM, SRC-3, p/CIP)
Description:
ACTR (also named AIB1, RAC3, and TRAM1; named SRC-3 and p/CIP in the mouse) is a member of the p160/SRC coactivator family (17, 20, 30, 32). Like other p160 coactivators, ACTR/AIB1 was identified as a nuclear cofactor that associates with hormone-bound nuclear receptors and mediates the transcriptional activation function of the receptors. Structural and functional studies revealed that the p160s contain functional domains for histone acetyltransferase activity and interactions with ligand-bound receptors, the coregulator proteins CBP and p300, PCAF, and arginine methyltransferase CARM1. Evidence from biochemical studies suggests that the association with the above-mentioned cofactors is important for the p160s to mediate the transactivation by nuclear receptors. It is generally conceived that the p160s are recruited to hormone-responsive genes through their interaction with activated receptors and then nucleate the assembly of a coactivator complex, which in turn remodels chromatin through histone modifications and facilitates RNA polymerase II transcription. The role of ACTR/AIB1 as well as the other p160s in animal development and physiology has been explored by genetic approaches. Similar to the other p160 knockouts, p/CIP/SRC-3 knockout mice exhibited abnormal development and reduced function of their reproductive systems. Unlike the SRC-1 knockout mice, however, animals with the SRC-3/p/CIP gene deleted displayed significant somatic growth retardation and a reduced capacity of growth factor-stimulated cell proliferation, although the underlying molecular mechanism is unclear.

Applications:
Suitable for use in Western Blot and Immunopreciptation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250-1:500; Detects ACTR/AIB1 in RIPA lysates from MCF-7 cells. 1:1000 dilution of a previous lot detected ACTR/AIBI in RIPA lysates from BG-1 ovarian cancer cells. BG-1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A0857. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 10ul immunoprecipitates ACTR/AIB1 from 500ug of BG-1 ovarian cancer cell RIPA lysate.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG0.T.198Ascites
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
Not determined
Immunogen:
GST fusion protein containing aa605-1294 of human ACTR/AIB1.
Purity:
Ascites
Form
Supplied as liquid in PBS, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes human ACTR/AIB1 at 150kD and a doublet at ~110kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Klinge, C.M. et al., (2004) J. Molecular Endocrinology (2004) 33:387-410. 1. Chen, H., et al., Cell 90: 569-580 (1997). 2. Anzik, S.L., et al., Science 277: 965-968 (1997). 3. Shang, Y., et al., Science 295: 2465-2468 (2002).