Technical Data
AIB 1 (Amplified in Breast Cancer-1)
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Amplified in breast cancer-1 (AIB-1), is a member of a growing family of transcriptional coactivator proteins which includes steroid receptor coactivator-1 (SRC-1) and glucocorticoid receptor interacting protein-1 (GRIP1). AIB1:RAC3 has been shown to interact and stimulate transcriptional activity of the retinoic acid, retinoid X, vitamin D, mineralocorticoid, glucocorticoid, thyroid hormone, androgen, progesterone, and peroxisome proliferator activated receptors. AIB1:RAC3 also interacts with the transcriptional integrators CREB binding protein (CBP) and p300.

Western Blot

Recommended Dilution:
Western Blot: Predicted molecular weight: 155kD. Can be blocked with AIB 1 peptide.
Optimal dilution to be determined by the researcher.

Positive Control:
T47D cell lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide: GLGENLDPLASDSC, corresponding to amino acids 3-15 of Human AIB 1.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
Detects human amplified in breast cancer 1 (AIB1). Species Crossreactivity: Expected to crossreact with Xenopus laevis (92% identity with immunogen) due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Anzick SL et al. AIB1, a steroid receptor coactivator amplified in breast and ovarian cancer. Science 277:965-8 (1997). 2. Li H et al. RAC3, a steroid/nuclear receptor-associated coactivator that is related to SRC-1 and TIF2. Proc Natl Acad Sci U S A 94:8479-84 (1997). 3. Takeshita A et al. TRAM-1, A novel 160kD thyroid hormone receptor activator molecule, exhibits distinct properties from steroid receptor coactivator-1. J Biol Chem 272:27629-34 (1997).