Technical Data
A0857-07
AIB 1 (Amplified in Breast Cancer-1)
Description:
AIB1-AC3 recognizes AIB1, a member of the SRC-1 family of nuclear receptor coactivators with an apparent molecular weight of 160kD. The AIB1 gene has been mapped to region 20q12 and has been shown to be amplified in breast tumors and pancreatic cancer cell lines. AIB 1 interacts in a ligand-dependent fashion with the estrogen receptor to enhance estrogen-dependent transcriptional gene activation.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1-5 mg/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k4i291Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide to the C-terminal end of human AIB1 conjugated to KLH. Molecular weight: 160kD.
Purity:
Purified by Protein G chromatography. ~95% as determined by SDS-PAGE.
Form
Supplied as a liquid in PBS, pH 7.4 before the addition of glycerol to 40%.
Specificity:
Recognizes human AIB 1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Anzick, S.L., et al. (1997) AIB1, a steroid receptor coactivator amplified in breast and ovarian cancer. Science 277: 965-968. 2.Bautista, S, et al. (1998) In breast cancer, amplification of the steroid receptor coactivator gene AIB1 is correlated with estrogen and progesterone receptor positivity. Clin Cancer Res 4: 2925-9. 3.Ghadimi, B.M., et al. (1999) Specific chromosomal aberrations and amplification of the AIB1 nuclear receptor coactivator gene in pancreatic carcinomas. Am J Pathol 154: 525-36.