Technical Data
ACTR1A (ARP1, Actin-Related Protein 1 Homolog A, Centractin alpha (yeast), Actin-RPV, Alpha-Centractin, ARP1, Centractin, Centrosome-associated Actin Homolog, CTRN1)
The actin-related protein ACTR1A, a member of the superfamily of actin-related proteins, is the major subunit of the dynactin complex, a key component of the cytoplasmic dynein motor machinery. The major components of the dynactin complex, consisting of at least ten polypeptides, include ACTR1A. ACTR1A, the most abundant subunit in the dynactin complex, is most similar to conventional actin (>50% amino acid identity). The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dyneinmediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1A overexpression in cells results in aberrant spindle morphologies and cell cycle delay at prometaphase, suggesting a possible function of ACTR1A/dynactin complex in progression through the prometaphase of mitosis.

Cellular Localization: Cytoplasmic

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:8000.
Western Blot: 1-3ug/ml. Predicted molecular weight 43kD. Detects a band of ~ 45kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide: YEEDGARSIHRKT representing the C-terminus of the human protein (residues 363-375) according to NP_005727.
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA, 0.02% sodium azide.
This antibody may crossreact with ACTR1B Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse (100% identity with immunogen) and Rat due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Lees-Miller JP et al. A vertebrate actin-related protein is a component of a multisubunit complex involved in microtubule-based vesicle motility. Nature 359:244-6 (1992).