Technical Data
A0858-98D
ADAM 8 (ADAM8, a Disintegrin and Metalloproteinase Domain 8, MS2, CD156, CD156 MS2)
Description:
Members of ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. The extracellular region of ADAM8 shows significant amino acid sequence homology to hemorrhagic snake venom proteins, including the metalloprotease and disintegrin domains. The expression of ADAM8 is upregulated by retinoic acid and vitamin D3. ADAM8 is involved in protein processing at the cell surface and is also thought to act as a leukocyte differentiation marker.

Cellular Localization: Cell Membrane

Applications:
Suitable for use in RIA, Immunofluorescence, Flow Cytometry, Direct ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1.0-3.0ug/ml. ~ 90kD band seen in Human Brain lysate.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide QRKQGAGAPTAP, representing the C-Terminus of the human protein (residues 813-824), according to NP_001100.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.
Specificity:
Species Crossreactivity: Crossreacts with Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Yoshiyama K et al. CD156 (human ADAM8): expression, primary amino acid sequence, and gene location. Genomics 41:56-62 (1997).