Technical Data
ADAM 10, CT (A Disintegrin And Metalloproteinase 10, MADM, Kuzbanian)
Proinflammatory cytokine tumor necrosis factor-a (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta. The genes encoding human, mouse and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-1:2000
Immunohistochemistry: 2ug/ml
Optimal dilution to be determined by the researcher.

Positive Control:
Jurkat whole cell lysate (85kD band).

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGHighly Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa732-748 of human ADAM10.
Purified by ion-exchange chromatography.
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes human ADAM10 (CT). Species Sequence Homology: bovine and rat: 100%; mouse: 94%
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rosendahl, M.S., et al., J. Biol. Chem. 272: 24,588-24,593 (1997). 2. Pan, D. & Rubin, G.M., Cell 90: 271-280 (1997). 3. Qi, H., et al., Science 283: 91-94 (1999). 4. Howard, L., et al., Biochem. J. 317: 45-50 (1996).