Technical Data
A0859-10G
ADAM 9 (A Disintegrin and Metalloproteinase Domain 9, ADAM Metallopeptidase Domain 9, ADAM9, ADAM-9, Cellular Disintegrin-related Protein, Disintegrin and Metalloproteinase Domain-containing Protein 9, KIAA0021, MDC9, Meltrin gamma, MLTNg, Metalloprotease
Description:
The ADAM (A Disintegrin and A Metalloprotease) family of multidomain membrane proteins influences cell signaling and adhesion by shedding cell surface proteins such as cytokines and growth factors, by influencing cell adhesion to the extracellular matrix (ECM), and by directly remodeling the ECM. Conserved domains in ADAM family members include a prodomain, a zinc-dependent metalloprotease domain, a disintegrin domain, a cysteine-rich domain, an EGF-like sequence and a short cytoplasmic tail (1,2).The prodomain is thought to aid in protein folding. Disintegrin and cysteine-rich domains mediate adhesion at least in part through binding to integrins. Phosphorylation of the cytoplasmic tail as well as its interaction with other signaling proteins may influence intra- and extracellular signaling (1). ADAM9 is widely distributed and has been shown to affect migration in skin keratinocytes (3,4). ADAM9 is overexpressed in prostate cancer (5), pancreatic cancer (6), gastric cancer (7) and has been linked to invasion and metastasis in small cell lung cancer (8). An alternatively spliced short (50 kDa) form of ADAM9 containing protease activity is involved in tumor cell invasion (9).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to the carboxy terminus of human ADAM9, conjugated to KLH (Q13443).
Purity:
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Recognizes endogenous levels of total ADAM9 protein, unprocessed and active forms. Does not recognize the carboxy terminally truncated short form of ADAM9. In some cell types, the antibody cross-reacts with a 50kD band of unknown origin. Species crossreactivity: mouse, rat and monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. N. M. Hooper and U. Lendeckel. The Adam Family Of Proteases. The Netherlands: Springer, 2005 2. Schlöndorff, J. and Blobel, C.P. (1999) J Cell Sci 112 ( Pt 21), 3603-17. 3. Franzke, C.W. et al. (2002) EMBO J 21, 5026-35. 4. Zigrino, P. et al. (2007) J Biol Chem 282, 30785-93. 5. Fritzsche, F.R. et al. (2008) Eur Urol 54, 1097-106. 6. Grützmann, R. et al. (2004) Br J Cancer 90, 1053-8. 7. Carl-McGrath, S. et al. (2005) Int J Oncol 26, 17-24. 8. Shintani, Y. et al. (2004) Cancer Res 64, 4190-6. 9. Mazzocca, A. et al. (2005) Cancer Res 65, 4728-38.