Technical Data
A0859-17A
ADAM10, Recombinant, Human, Western Blot Positive Control (Disintegrin and Metalloproteinase Domain-containing Protein 10, ADAM 10, CDw156, Kuzbanian Protein Homolog, Mammalian Disintegrin-metalloprotease, CD156c, KUZ, MADM)
10ug
Molecular Biology Storage: -20CShipping: Blue Ice
ADAM 10 is a member of the ADAM family of membrane-anchored cell surface proteins, which contain both a disintegrin and a metalloprotease domain. ADAM 10 is expressed in a wide variety of tissues and resides on both the cell surface and within the cytoplasm. ADAM10 has been implicated in proteolytic release of a number of cell surface proteins in particular TNF alpha.

Source:
Recombinant corresponding to aa214-672 from human ADAM10, fused to N-terminal 6x His-tag, expressed in E. coli.

Applications:
Suitable for use in Western Blot as a control. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: Purified (SDS-PAGE)
Concentration: As reported
Form: Supplied as a liquid in 25mM Tris-HCl, pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue, 2.5% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Primakoff, P. & Myles D.G. (2000) Trends Genetics 16 (2): 83-87.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.