Technical Data
A0859-20
ADAM 10 (Kuzbanian, KUZ, MADM, a Disintegrin and Metalloprotease 10)
Description:
ADAM10 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. It cleaves the membrane-bound precursor of TNF-alpha at 76-Ala-|-Val-77 to its mature soluble form. It is responsible for the proteolytic release of several cell-surface proteins, including heparin binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). It contributes to the normal cleavage of the cellular prion protein and is involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicules, suggesting a vesicle-based protease activity.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:500-1:2000 detected ADAM 10 on 10ug of RAW264.7 lysates. RAW264.7 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with A0859-20 (1:1000). Proteins were visualized using an IgG (HRP) donkey anti-rabbit and chemiluminesence detection system.
Immunohistochemistry: A previous lot of this antibody was used to detect ADAM10 in formalin-fixed, paraffin-embedded renal tissue and renal cell carcinoma tissue (Gutwein, P., et al., 2009).
Immunofluorescence: A previous lot of this antibody was used to detect ADAM10 in RCC4 and A498 cells (Gutwein, P., et al., 2009).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Raised against a peptide corresponding to aa732-748 of human ADAM10. This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid. Species Sequence Homology: rat
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human ADAM 10, C-terminus. Species Crossreactivity: bovine and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gutwein, P., et al. EUROPEAN JOURNAL OF CANCER. 45(2009):478489. 2. Farris W., et al., (2003). PNAS. 100 (7):4162- 4167. 3. Slack B.E., et al. (2001). Biochem. J. 357:787- 794. 4. Qi, H., et al. (1999). Science. 283:91-4. 5. Pan, D. (1997). Cell. 90: 271-80. 6. Rosendahl, M.S. (1997). J. Biol. Chem. 272:24588-93. 7. Howard, L., et al. (1996). Biochem J. 317:45