Technical Data
A0859-20
ADAM 10 (Kuzbanian, KUZ, MADM, a Disintegrin and Metalloprotease 10)
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Technical Data |
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A0859-20 |
ADAM 10 (Kuzbanian, KUZ, MADM, a Disintegrin and Metalloprotease 10) |
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Description: Tumor-necrosis factor alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a Tcell lymphoma. TNA-alpha, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloproteinase domain), was recently identified to cleave TNF-alpha, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM10, kuzbanian (KUZ), and MADM. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilution: Western blot: 1:500 – 1:2,000 dilution. Jurkat whole cell lysate can be used as a positive control and an 85kD band can be detected which may represent precursor. A 60kD faint band was detected in some cell lines including Jurkat, appearing to be the processed mature protein. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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