Technical Data
A0859-20A
ADAM 10 (Kuzbanian, KUZ, MADM, a Disintegrin and Metalloprotease 10)
Description:
ADAM10 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. It cleaves the membrane-bound precursor of TNF-alpha at 76-Ala-|-Val-77 to its mature soluble form and is responsible for the proteolytic release of several cell-surface proteins, including heparin binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). It contributes to the normal cleavage of the cellular prion protein and is involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicules, suggesting a vesicle-based protease activity.

Cellular Localization: Cell membrane, Type I membrane protein. Also localized in the Golgi apparatus.

Positive Control: Jurkat whole cell lysate

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:2000. A band at 85kD. A 60kD faint band can be detected insome cell lines including Jurkat, which appears to be the processed mature protein.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide, corresponding to amino acids 732-748 of Human ADAM10. This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid. Epitope: PQRQRPRESYQMGHMRR
Purity:
Purified by immunoaffinity chromatography.
Form
PBS with 0.02% sodium azide
Specificity:
Species Crossreactivity: Crossreacts with Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Qi H et al. Processing of the notch ligand delta by the metalloprotease Kuzbanian. Science 283:91-4 (1999). Rosendahl MS et al. Identification and characterization of a pro-tumor necrosis factor-alpha-processing enzyme from the ADAM family of zinc metalloproteases. J Biol Chem 272:24588-93 (1997). Pan D & Rubin GM Kuzbanian controls proteolytic processing of Notch and mediates lateral inhibition during Drosophila and vertebrate neurogenesis. Cell 90:271-80 (1997). Howard L et al. Molecular cloning of MADM: a catalytically active mammalian disintegrin-metalloprotease expressed in various cell types. Biochem J 317 (Pt 1):45-50 (1996).