		Technical Data
A0859-35G	ADAM 17, Propeptide Domain (A Disintegrin And Metalloproteinase 17, TNF-a Converting Enzyme, TACE, TNF alpha Converting Enzyme, CD156b)

Description:
TACE (TNF-alpha converting enzyme) protein is a membrane bound matalloprotease from ADAM (a disintegrin and metalloprotease) family of proteins. It cleaves 26kD type II membrane-bound precursor of TNF-a into 17kD mature form which is secreted. TACE is also known to process other membrane proteins including TNF receptor, TGFa, L-selectin and amyloid protein precursor.

Epitope: C-terminal

Applications:
Western Blot: (Ab 5ug/ml for 2 hrs at RT)
Immunoprecipitation: (Denatured verified) (Use Protein A) (Ab 10ug/mg protein lysate)

Positive Control: HeLa cells

Cellular Localization: Cell membrane

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
250ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

A synthetic peptide from the C-terminus of human TACE protein.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide.

Specificity:

Recognizes TACE. Species Crossreactivity: Human, Mouse and Rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Moss M L, et al (1997). Nature 385: 733-736. 2. Black R A, et al. (1997) Nature 385: 729-733.