Technical Data
ADAM 17, Propeptide Domain (A Disintegrin And Metalloproteinase 17, TNF-a Converting Enzyme, TACE, TNF alpha Converting Enzyme, CD156b)
TACE (TNF-alpha converting enzyme) protein is a membrane bound matalloprotease from ADAM (a disintegrin and metalloprotease) family of proteins. It cleaves 26kD type II membrane-bound precursor of TNF-a into 17kD mature form which is secreted. TACE is also known to process other membrane proteins including TNF receptor, TGFa, L-selectin and amyloid protein precursor.

Epitope: C-terminal

Western Blot: (Ab 5ug/ml for 2 hrs at RT)
Immunoprecipitation: (Denatured verified) (Use Protein A) (Ab 10ug/mg protein lysate)

Positive Control: HeLa cells

Cellular Localization: Cell membrane

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
250ul-20CBlue IceHumanRabbit
A synthetic peptide from the C-terminus of human TACE protein.
Purified by Protein A affinity chromatography.
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide.
Recognizes TACE. Species Crossreactivity: Human, Mouse and Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Moss M L, et al (1997). Nature 385: 733-736. 2. Black R A, et al. (1997) Nature 385: 729-733.