Technical Data
ADAM 19 (A Disintegrin And Metalloproteinase 19) (Biotin)
ADAM19 (a disintegrin and metalloprotease 19; also MADDAM and Meltrin- ) is a
95-100kD member of the M12B peptidase family of enzymes. It is expressed on
multiple cell types including monocytes, fibroblasts, osteoblasts and dendritic cells. The
human ADAM19 proenzyme is a 931 amino acid (aa) type I transmembrane protein. It
contains a 178 aa cleavable proregion (aa 26-203) and a 497 aa extracellular domain
(ECD) (aa 204-702). The ECD contains a metalloprotease domain (aa 211-409), a
disintegrin region (aa 417-503) and a Cys-rich domain (aa 504-650). The ECD
circulates in plasma bound to 700kD 2-Macroglobulin. One splice variant shows an
alternate start site at Met71, while two isoforms show variations afterLys902. Over
aa 205-702, human ADAM19 is 87% aa identical to mouse ADAM19.

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanSheep
CHO cell-derived, rhADAM19; aa 26-501; with a Ser32 deletion and a Lys33Arg substitution.
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Recognizes human ADAM19.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.