Technical Data
A0859-42A
ADAM 33 (A Disintegrin and Metalloprotease 33, A Disintegrin and Metalloproteinase Domain 33, HGNC:15478, DJ964F7.1, DKFZp434K0521, FLJ35308, FLJ36751, MGC71889, Disintegrin and Reprolysin Metalloproteinase Family Protein, Metalloprotease Disintegrin)
Description:
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This protein is a type I transmembrane protein implicated in asthma and bronchial hyperresponsiveness. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:64,000.
Western Blot: 0.1-0.3ug/ml. Detected a band of ~ 80-90kD and strong ladder from 26 to 38kD after 1ug/ml antibody staining. This protein has a calculated MW of 87.7kD according to NP_079496.1.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide: CQSRRCRKNAFQEL, representing the internal region of the human protein according to NP_079496.1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Species Crossreactivity: Crossreacts with Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Blakey J, Halapi E, Bjornsdottir US, Wheatley A, Kristinsson S, Upmanyu R, Stefansson K, Hakonarson H, Hall IP. Contribution of ADAM33 polymorphisms to the population risk of asthma. Thorax. 2005 Apr;60(4):274-6.