		Technical Data
A0859-43	ADAP (SLAP-130, Fyb, FYN Binding Protein, Adhesion and Degranulation Promoting Adaptor Protein, B630013F22Rik, FYB-120/130, FYN-binding Protein, FYN-T-binding Protein, p120/p130, SLP-76-associated Phosphoprotein)

Description:
FYB (FYN binding protein) was initially identified as a molecular adaptor that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of FYB, ultimately activates the NFkB family of transcription factors. More recent experiments have shown that FYB controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bclx(L), suggesting that FYB regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of FYB are known to exist.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5-2.0ug/ml detected ADAP/SLAP-130/Fyb in RIPA lysates from RAW 264.7 macrophage cells. RAW 264.7 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A0859-43. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitated ADAP/SLAP-130/Fyb from 500ug of RAW 264.7 macrophage RIPA lysate.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40J: IgG, Fc (HRP) Pab Gt x Rb

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice	Mouse	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to aa755-768 DNDGEIYDDIADGC of mouse Fyn binding protein (ADAP/SLAP-130/Fyb) (KLH).

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

Specificity:

Recognizes mouse ADAP/SLAP-130/Fyb, Mr ~130kD. An unknown protein was also detected, Mr ~40kD that is also inhibited by the immunizing peptide. Species Sequence Homology: human and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Liping, G., et al., Proc. Natl. Acad. Sci. 98: 11,527-11,532 (2001). 2. Boerth, N.J., et al., J. Biol. Chem. 275: 5143-5152 (2000). 3. Hunter, A.J., et al., J. Immunol. 164: 1143-1147 (2000).