Technical Data
A0859-43C
ADAP (SLAP-130, Fyb)
Description:
Human SH2 domain containing leukocyte protein-76-Associated Phosphoprotein of 130kD (SLAP-130), also known as Fyb-120 and ADAP, is an 86kD, 783 amino acid, cytoplasmic protein that runs anomalously as a 120-130kD protein in SDS-PAGE. Human SLAP-130 is a hematopoietic-specific adaptor that associates with other cytoplasmic proteins such as SH2-containing Leukocyte Phosphoprotein of 76kD (SLP-76). Adaptors integrate signals from surface receptors and are essential components of many immune cell-signaling cascades. There is at least one known alternatively spliced SLAP-130 isoform which contains an additional 46 aa. Over the region used as immunogen, SLAP-130 is 66% and 76% identical to the corresponding canine and mouse proteins, respectively.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS, 01% sodium azide. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG38H202Affinity Purified
SizeStorageShippingSourceHost
100ug4C Do Not FreezeBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Recombinant human SLAP-130 aa 2-274 (E. coli).
Purity:
Purified by Protein G chromatography.
Form
Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with 100ul 40-50% glycerol, PBS, 01% sodium azide.
Specificity:
Recognizes human SLAP-130.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.