Technical Data
A0859-43E
ADAP (SLAP-130, Fyb)
Description:
FYN-binding protein (FYB) is an adapter protein for Fyn and SLP-76 signaling cascades in T cells. A hematopoietic protein, FYB associates with p59fyn and becomes tyrosine-phosphorylated in response to receptor ligation. FYB has been associated to modulation of IL-2 expression in T cells and platelet activation (3). Fyn (p59Fyn) is a 59kDa member of the Src family of tyrosine kinases. The carboxy terminus of Fyn shares extensive amino acid sequence homology with Src, but is very different within the amino-terminal 81 amino acid residues. The Fyn protein is synthesized and N-myristoylated on cytosolic polysomes and then rapidly targeted to the plasma membrane, where it is palmitoylated. Fyn's biological functions are diverse and include signaling via the T cell receptor, regulation of brain function and adhesion mediated signaling (2, 3). FYN-binding protein (FYB) is an adapter protein for Fyn and SLP-76 signaling cascades in T cells. A hematopoietic protein, FYB associates with p59fyn and becomes tyrosine-phosphorylated in response to receptor ligation. FYB has been associated to modulation of IL-2 expression in T cells and platelet activation (3).

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500
Immunohistochemistry: 1:100-250
Immunocytochemistry: 1:100-250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E123Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues on the C-terminus of human FYB.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Specificity:
Recognizes human FYB
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Renzoni D.A., et al; Biochemistry 35:15646-1565, 1996. 2. Mustelin Tet al; Eur J Immunol. 1992 May;22(5):1173-8. 3. Silva AJ, et al. PNAS USA, 94(14):7493-7498, 1997.