Technical Data
A0859-44C
Adaptor Protein, Phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1, DIP13alpha, Signaling Adaptor Protein DIP13alpha, adaptor protein containing pH Domain, PTB domain and leucine zipper motif)
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Technical Data |
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A0859-44C |
Adaptor Protein, Phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1, DIP13alpha, Signaling Adaptor Protein DIP13alpha, adaptor protein containing pH Domain, PTB domain and leucine zipper motif) |
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Description: APPL1, an adaptor protein containing an NH2-terminal Bin/Amphiphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain and a COOH-terminal phosphotyrosine binding (PTB) domain, was originally identified as an interacting partner of Akt in a yeast two-hybrid assay using Akt2 as a bait. APPL1 binds to a number of cell surface receptors (TrkA, DCC, adiponectin, FSH) and intracellular signaling molecules (small GTPase Rab5, GIPC and inositol 5- phosphatase, suggesting that APPL1 may act as a common relay to coordinate diverse signaling cascades. APPL1 potentiates insulin-mediated Akt activation by counteracting the effect of the Akt inhibitor TRB3. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA:0.5-1ug/ml Western Blot:0.1-0.2ug/ml Immunohistochemistry:1-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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