Technical Data
Adaptor Protein, Phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1, DIP13alpha, Signaling Adaptor Protein DIP13alpha, adaptor protein containing pH Domain, PTB domain and leucine zipper motif)
APPL1, an adaptor protein containing an NH2-terminal Bin/Amphiphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain and a COOH-terminal phosphotyrosine binding (PTB) domain, was originally identified as an interacting partner of Akt in a yeast two-hybrid assay using Akt2 as a bait. APPL1 binds to a number of cell surface receptors (TrkA, DCC, adiponectin, FSH) and intracellular signaling molecules (small GTPase Rab5, GIPC and inositol 5- phosphatase, suggesting that APPL1 may act as a common relay to coordinate diverse signaling cascades. APPL1 potentiates insulin-mediated Akt activation by counteracting the effect of the Akt inhibitor TRB3.

Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot:0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Recombinant full-length human APPL1 expressed in E.coli.
Purified by immunoaffinity chromatography.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA.
Recognizes human APPL1. Species Cross-Reactivity: monkey, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.